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Journal: Translational Oncology
Article Title: CCL22 as an independent prognostic factor in endometrial cancer patients
doi: 10.1016/j.tranon.2024.102116
Figure Lengend Snippet: Representative images of CCL22-expression in EC: (A) intermediate expression in glandular cells (IRS=2.67), S/M (IRS=0.67); (B) high expression in glandular cells (IRS=12.00), S/M (IRS=5.33); (C) strongly positive cells in tumor distant S/M; (D) significant correlation between CCL22 in tumor- and stroma-cells; (E) CCL22 levels in tumor cells were higher than in S/M. Objective 20x, Scale bar 100 µm.
Article Snippet: Paraffin-embedded TMA of EC-patients and tissue of the control group were incubated with the
Techniques: Expressing
Journal: Translational Oncology
Article Title: CCL22 as an independent prognostic factor in endometrial cancer patients
doi: 10.1016/j.tranon.2024.102116
Figure Lengend Snippet: Representative images of CCL22-expression in endometrial control with negative (A) and strong staining in glandular epithelial cells (B) and strongly positive cells in myometrium (C). Elevated CCL22-expression in glandular epithelial cells compared to stroma cells (D). Significantly lower CCL22-IRS EC compared to benign endometrium (E). Concerning CCL22 in S/M a trend to higher levels was found in the specimens of EC patients (F). Objective 20x, Scale bar 100 µm.
Article Snippet: Paraffin-embedded TMA of EC-patients and tissue of the control group were incubated with the
Techniques: Expressing, Control, Staining
Journal: Translational Oncology
Article Title: CCL22 as an independent prognostic factor in endometrial cancer patients
doi: 10.1016/j.tranon.2024.102116
Figure Lengend Snippet: CCL22-expression in S/M increases with higher grade in EC (A). High CCL22-expression in S/M in EC is associated to poorer OS (B), but not to PFS (C). (D) High CCL22-expression in tumor epithelium is associated to prolonged OS, but not to PFS (E).
Article Snippet: Paraffin-embedded TMA of EC-patients and tissue of the control group were incubated with the
Techniques: Expressing
Journal: Translational Oncology
Article Title: CCL22 as an independent prognostic factor in endometrial cancer patients
doi: 10.1016/j.tranon.2024.102116
Figure Lengend Snippet: Isolated CCL22+ cells were identified as mainly M1-macrophages in distant myometrial tissue areas by double immunofluorescence: (A) Representative EC stained for CCL22 (red) and CD68, CD80, CD163, and DEC205 (green); (B) Proportion of CCL22+ cells, that expressed also one of those immune cells markers is presented; (C) Proportion of total expression of all markers: dominant occurrence of CD68+ and CD80+ cells; (D) Kaplan-Meier Curve for OS and PFS (E). Correlation analysis of FoxP3 and CCL22 in S/M (F), distant strongly positive cells (G), and in tumor cells (H).
Article Snippet: Paraffin-embedded TMA of EC-patients and tissue of the control group were incubated with the
Techniques: Isolation, Immunofluorescence, Staining, Expressing
Journal: Translational Oncology
Article Title: CCL22 as an independent prognostic factor in endometrial cancer patients
doi: 10.1016/j.tranon.2024.102116
Figure Lengend Snippet: High CCL22 secretion by freshly isolated PBMCs in contrast to EC cell lines without stimulation (A). Significantly increased CCL22 in SN after coculture of PBMCs and EC cell lines (B). The addition of tumor-SN to PBMCs led to a significant increase of CCL22 levels (C), while incubation of tumor cells with PBMC-SN resulted in a decrease in CCL22 levels (D). mRNA levels of tumor cells after coculture revealed a significant increase in CCL22 of Ishikawa+ with PBMCs (E). CCL22 levels of tumor RIPA lysates also revealed a significant increase after coculture (F). All experiments were carried out in technical triplicate and repeated three times with PBMCs from different blood donors.
Article Snippet: Paraffin-embedded TMA of EC-patients and tissue of the control group were incubated with the
Techniques: Isolation, Incubation